Author dc.contributor.author | Laurinyecz, Barbara | |
Author dc.contributor.author | Vedelek, Viktor | |
Author dc.contributor.author | Kovács, Attila L | |
Author dc.contributor.author | Szilasi, Kinga | |
Author dc.contributor.author | Lipinszki, Zoltán | |
Author dc.contributor.author | Slezák, Csilla | |
Author dc.contributor.author | Darula, Zsuzsanna | |
Author dc.contributor.author | Juhász, Gábor | |
Author dc.contributor.author | Sinka, Rita | |
Availability Date dc.date.accessioned | 2022-07-22T10:06:22Z | |
Availability Date dc.date.available | 2022-07-22T10:06:22Z | |
Release dc.date.issued | 2019 | |
uri dc.identifier.uri | http://hdl.handle.net/10831/67045 | |
Abstract dc.description.abstract | Drosophila melanogaster sperm reach an extraordinary long size, 1.8 mm, by the end of spermatogenesis. The mitochondrial derivatives run along the entire flagellum and provide structural rigidity for flagellar movement, but its precise function and organization is incompletely understood. The two mitochondrial derivatives differentiate and by the end of spermatogenesis the minor one reduces its size and the major one accumulates paracrystalline material inside it. The molecular constituents and precise function of the paracrystalline material have not yet been revealed. Here we purified the paracrystalline material from mature sperm and identified by mass spectrometry Sperm-Leucylaminopeptidase (S-Lap) family members as important constituents of it. To study the function of S-Lap proteins we show the characterization of classical mutants and RNAi lines affecting of the S-Lap genes and the analysis of their mutant phenotypes. We show that the male sterile phenotype of the S-Lap mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives. Our work shows that S-Lap proteins localize and accumulate in the paracrystalline material of the major mitochondrial derivative. Therefore, we propose that S-Lap proteins are important constituents of the paracrystalline material of Drosophila melanogaster sperm. | |
Language dc.language | Angol | |
dc.rights | Nevezd meg! CC BY | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
Title dc.title | Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm. | |
Type dc.type | folyóiratcikk | |
Date Change dc.date.updated | 2022-05-13T13:04:31Z | |
Note dc.description.note | Funding Agency and Grant Number: National Research, Development and Innovation Office of Hungary [GINOP-2.3.2-15-2016-00032, 2.3.2-15-2016-00035, GINOP-2.3.2-15-2016-00001, EFOP-3.6.1-16-2016-00008, NF101001, GINOP 2.3.2-15-2016-00034]; Hungarian Academy of Sciences [LP2017-7/2017] Funding text: This work was funded by National Research, Development and Innovation Office of Hungary (http://nkfih.gov.hu/) GINOP-2.3.2-15-2016-00032 to GJ, 2.3.2-15-2016-00035, GINOP-2.3.2-15-2016-00001, EFOP-3.6.1-16-2016-00008, NF101001 to RS, and GINOP 2.3.2-15-2016-00034 and Hungarian Academy of Sciences Lendulet Program Grant LP2017-7/2017 to ZL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Összes idézések száma a WoS-ban: 0 Department of Genetics, University of Szeged, Szeged, Hungary Department of Anatomy, Eötvös Loránd University, Budapest, Hungary Institute of Biochemistry and MTA SZBK Lendület Laboratory of Cell Cycle Regulation, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Cited By :3 Export Date: 20 July 2020 Correspondence Address: Sinka, R.; Department of Genetics, University of SzegedHungary; email: rsinka@bio.u-szeged.hu Department of Genetics, University of Szeged, Szeged, Hungary Department of Anatomy, Eötvös Loránd University, Budapest, Hungary Institute of Biochemistry and MTA SZBK Lendület Laboratory of Cell Cycle Regulation, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Cited By :6 Export Date: 13 January 2021 Correspondence Address: Sinka, R.; Department of Genetics, University of SzegedHungary; email: rsinka@bio.u-szeged.hu | |
Doi ID dc.identifier.doi | 10.1371/journal.pgen.1007987 | |
Wos ID dc.identifier.wos | 000459970100056 | |
ID Scopus dc.identifier.scopus | 85062195702 | |
MTMT ID dc.identifier.mtmt | 30481567 | |
Issue Number dc.identifier.issue | 2 | |
abbreviated journal dc.identifier.jabbrev | PLOS GENET | |
Journal dc.identifier.jtitle | PLOS GENETICS | |
Volume Number dc.identifier.volume | 15 | |
Release Date dc.description.issuedate | 2019 | |
Pubmed ID dc.identifier.pubmed | 30802236 | |
department of Author dc.contributor.institution | Genetikai Intézet | |
department of Author dc.contributor.institution | Lendület Drosophila Autofágia Kutatócsoport | |
department of Author dc.contributor.institution | Genetikai Tanszék | |
department of Author dc.contributor.institution | Központi Laboratóriumok | |
department of Author dc.contributor.institution | Biokémiai Intézet | |
department of Author dc.contributor.institution | Anatómiai, Sejt- és Fejlődésbiológiai Tanszék | |
Author institution dc.contributor.department | Genetikai Tanszék | |
Author institution dc.contributor.department | Genetikai Tanszék | |
Author institution dc.contributor.department | Anatómiai, Sejt- és Fejlődésbiológiai Tanszék | |
Author institution dc.contributor.department | Genetikai Tanszék | |
Author institution dc.contributor.department | Biokémiai Intézet | |
Author institution dc.contributor.department | Genetikai Tanszék | |
Author institution dc.contributor.department | Biokémiai Intézet | |
Author institution dc.contributor.department | Anatómiai, Sejt- és Fejlődésbiológiai Tanszék | |
Author institution dc.contributor.department | Genetikai Tanszék |
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