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Author
dc.contributor.author
Laurinyecz, Barbara 
Author
dc.contributor.author
Vedelek, Viktor 
Author
dc.contributor.author
Kovács, Attila L 
Author
dc.contributor.author
Szilasi, Kinga 
Author
dc.contributor.author
Lipinszki, Zoltán 
Author
dc.contributor.author
Slezák, Csilla 
Author
dc.contributor.author
Darula, Zsuzsanna 
Author
dc.contributor.author
Juhász, Gábor 
Author
dc.contributor.author
Sinka, Rita 
Availability Date
dc.date.accessioned
2022-07-22T10:06:22Z
Availability Date
dc.date.available
2022-07-22T10:06:22Z
Release
dc.date.issued
2019
uri
dc.identifier.uri
http://hdl.handle.net/10831/67045
Abstract
dc.description.abstract
Drosophila melanogaster sperm reach an extraordinary long size, 1.8 mm, by the end of spermatogenesis. The mitochondrial derivatives run along the entire flagellum and provide structural rigidity for flagellar movement, but its precise function and organization is incompletely understood. The two mitochondrial derivatives differentiate and by the end of spermatogenesis the minor one reduces its size and the major one accumulates paracrystalline material inside it. The molecular constituents and precise function of the paracrystalline material have not yet been revealed. Here we purified the paracrystalline material from mature sperm and identified by mass spectrometry Sperm-Leucylaminopeptidase (S-Lap) family members as important constituents of it. To study the function of S-Lap proteins we show the characterization of classical mutants and RNAi lines affecting of the S-Lap genes and the analysis of their mutant phenotypes. We show that the male sterile phenotype of the S-Lap mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives. Our work shows that S-Lap proteins localize and accumulate in the paracrystalline material of the major mitochondrial derivative. Therefore, we propose that S-Lap proteins are important constituents of the paracrystalline material of Drosophila melanogaster sperm.
Language
dc.language
Angol

dc.rights
Nevezd meg! CC BY

dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
Title
dc.title
Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm.
Type
dc.type
folyóiratcikk
Date Change
dc.date.updated
2022-05-13T13:04:31Z
Note
dc.description.note
Funding Agency and Grant Number: National Research, Development and Innovation Office of Hungary [GINOP-2.3.2-15-2016-00032, 2.3.2-15-2016-00035, GINOP-2.3.2-15-2016-00001, EFOP-3.6.1-16-2016-00008, NF101001, GINOP 2.3.2-15-2016-00034]; Hungarian Academy of Sciences [LP2017-7/2017] Funding text: This work was funded by National Research, Development and Innovation Office of Hungary (http://nkfih.gov.hu/) GINOP-2.3.2-15-2016-00032 to GJ, 2.3.2-15-2016-00035, GINOP-2.3.2-15-2016-00001, EFOP-3.6.1-16-2016-00008, NF101001 to RS, and GINOP 2.3.2-15-2016-00034 and Hungarian Academy of Sciences Lendulet Program Grant LP2017-7/2017 to ZL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Összes idézések száma a WoS-ban: 0 Department of Genetics, University of Szeged, Szeged, Hungary Department of Anatomy, Eötvös Loránd University, Budapest, Hungary Institute of Biochemistry and MTA SZBK Lendület Laboratory of Cell Cycle Regulation, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Cited By :3 Export Date: 20 July 2020 Correspondence Address: Sinka, R.; Department of Genetics, University of SzegedHungary; email: rsinka@bio.u-szeged.hu Department of Genetics, University of Szeged, Szeged, Hungary Department of Anatomy, Eötvös Loránd University, Budapest, Hungary Institute of Biochemistry and MTA SZBK Lendület Laboratory of Cell Cycle Regulation, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Cited By :6 Export Date: 13 January 2021 Correspondence Address: Sinka, R.; Department of Genetics, University of SzegedHungary; email: rsinka@bio.u-szeged.hu
Doi ID
dc.identifier.doi
10.1371/journal.pgen.1007987
Wos ID
dc.identifier.wos
000459970100056
ID Scopus
dc.identifier.scopus
85062195702
MTMT ID
dc.identifier.mtmt
30481567
Issue Number
dc.identifier.issue
2
abbreviated journal
dc.identifier.jabbrev
PLOS GENET
Journal
dc.identifier.jtitle
PLOS GENETICS
Volume Number
dc.identifier.volume
15
Release Date
dc.description.issuedate
2019
Pubmed ID
dc.identifier.pubmed
30802236
department of Author
dc.contributor.institution
Genetikai Intézet
department of Author
dc.contributor.institution
Lendület Drosophila Autofágia Kutatócsoport
department of Author
dc.contributor.institution
Genetikai Tanszék
department of Author
dc.contributor.institution
Központi Laboratóriumok
department of Author
dc.contributor.institution
Biokémiai Intézet
department of Author
dc.contributor.institution
Anatómiai, Sejt- és Fejlődésbiológiai Tanszék
Author institution
dc.contributor.department
Genetikai Tanszék
Author institution
dc.contributor.department
Genetikai Tanszék
Author institution
dc.contributor.department
Anatómiai, Sejt- és Fejlődésbiológiai Tanszék
Author institution
dc.contributor.department
Genetikai Tanszék
Author institution
dc.contributor.department
Biokémiai Intézet
Author institution
dc.contributor.department
Genetikai Tanszék
Author institution
dc.contributor.department
Biokémiai Intézet
Author institution
dc.contributor.department
Anatómiai, Sejt- és Fejlődésbiológiai Tanszék
Author institution
dc.contributor.department
Genetikai Tanszék


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Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm.
 

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Nevezd meg! CC BY
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